Product: Anti-Caspase-6 pAb
Cat. No.: PC-528 (100 μg)
Synonyms:
Anti-Mch2.
Background:
Caspases are a family of cysteine proteases that
can be divided into the apoptotic and
inflammatory caspase subfamilies. Unlike the
apoptotic caspases, members of the
inflammatory subfamily are generally not
involved in cell death but are associated with the
immune response to microbial pathogens. The
apoptotic subfamily can be further divided into
initiator caspases, which are activated in
response to death signals, and executioner
caspases, which are activated by the initiator
caspases and are responsible for cleavage of
cellular substrates that ultimately lead to cell
death. Caspase-6 is an executioner caspase
that was idientifed based on its homology with
human caspases 2 and 3 as well as the C.
elegans cell death protein CED-3. It possesses
two isoforms, of which only the longer form
possesses protease activity. Caspase-6 is highly
expressed in adult brain and may play a role in
several neuronal pathologies.
Specificity:
Recognizes Caspase-6. Depending on cell lines
or tissues used, either full-length or other
cleavage products may be observed.
Positive Control:
Jurkat cell lysate.
Species Reactivity:
Human. Other species not tested.
Host:
Rabbit
Immunogen:
A 15 amino acid peptide near the aminoterminus
of human Caspase-6 (GenBank
Accession No. NP_001217).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 μg/mL.

Immunocytochemistry: Use at ~2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of Caspase-6 in
Jurkat cell lysate with anti-Caspase-6 pAb at (A) 0.5
μg/mL, (B) 1 μg/mL and (C) 2 μg/mL.
Figure 2: Immunocytochemistry of Caspase-6 in
Jurkat cells with anti-Caspase-6 pAb at 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any damage,
personal injury, or economic loss caused by this
product.