Anti-BCRP mAb, clone BXP-21
Cat. No.: MC-236 (1 mL)
MC-177 (0.5 mL)
Specificity:
BXP-21 reacts with an internal epitope of Breast
Cancer Resistance Protein (BCRP, ABCG2), a
70 kDa transmembrane half-transporter which is
involved in multi-drug resistance. BXP-21 does
not cross-react with the human MDR1, MRP1,
MRP2 gene products.
Species Reactivity:
Reacts with human BCRP. Others not tested.
Ig Isotype:
Mouse IgG2a
Immunogen:
A fusion protein consisting of the E. coli maltosebinding
protein and a 126 amino acids part of
the BCRP peptide (aa 271-396 of BCRP,
GenBank accession no. AF098951).
Format:
1 mL (>200 tests) or 0.5 mL (>100 tests)
monoclonal antibody at ~250 μg IgG/mL in
serum-free culture supernatant with protein
stabilizer and 0.1% sodium azide.
Storage:
Store at 4°C for short term. Aliquot and store at
-20°C for long term. Avoid repeated freeze/thaw
cycles.
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Applications and Suggested Dilutions:

Immunocytochemistry: Use at a dilution of
1:20-1:50 on acetone-fixed cytospin
preparations.

Immunohistochemistry: Use at a dilution of
1:20 on acetone-fixed frozen sections,
followed by incubation with rabbit antimouse
IgG and a monoclonal mouse
APAAP complex. BXP-21 can be used on
formaldehyde-fixed and paraffin-embedded
human tissues and tumors: Pretreat with 10
mM citric acid, pH 6.0, for 20 min.

Western blot

Flow cytometry: Optimal conditions still to
be determined