Product: Anti-Clusterin mAb, clone CLI-9
Cat. No.: MC-495 (100 μg)
Synonyms:
Anti-TRPM-2, anti-Apolipoprotein J, anti-CLI,
anti-SGP-2, anti-CLU, anti-APO-J
Background:
Clusterin is the first identified extracellular
mammalian chaperone and appears to be
implicated in such diverse processes as sperm
maturation, regulation of complement activation,
programmed cell death, tissue remodeling and
lipid transport. Increases in clusterin mRNA and
protein have been consistently detected in
apoptotic cell death paradigms, establishing
clusterin gene expression as a prominent
marker of apoptotic cell loss. A dual role has
been shown for clusterin in that cells can be
protected from damage and dead cells can be
more easily removed after some types of cellular
damage.
Specificity:
Recognizes human clusterin.
Species Reactivity:
Human. Others not tested.
Ig Isotype:
Mouse IgG1
Immunogen:
Purified human clusterin (TRPM-2).
Format:
100 μg of liquid purified monoclonal antibody at
1 mg/mL in PBS containing 0.02% sodium azide.
Purity:
³ 95% as determined by SDS-PAGE.
Storage:
Store at 4°C. Do not freeze.
Applications and Suggested Dilutions:

Immunohistochemistry: Frozen sections.

Immunoprecipitation

Immunocytochemistry

Western blot: Use at approximately a 1:2,000
dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Detection of natural human clusterin in transfected
HEK 293T cells with MC-495.
Method: Endogenous human clusterin from cell lysates was
resolved by SDS-PAGE under reducing conditions,
transferred to nitrocellulose and incubated with a 1:2,000
dilution of MC-495. Proteins were visualized using an HRPconjugated
anti-mouse IgG antibody and a
chemiluminescence detection system.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.