FITC标记的磷酸化IRE1蛋白抗体
产品名称： FITC标记的磷酸化IRE1蛋白抗体
英文名称： Anti-phospho-IRE1 (Ser724)/FITC
产品编号： HZ-16698R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-phospho-IRE1 (Ser724)/FITC Conjugated antibody
FITC标记的磷酸化IRE1蛋白抗体
| 英文名称 | Anti-phospho-IRE1 (Ser724)/FITC |
| 中文名称 | FITC标记的磷酸化IRE1蛋白抗体 |
| 别 名 | IRE1 (phospho S724); p-IRE1 (phospho S724); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 107kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway. Function: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed. High levels observed in pancreatic tissue. Post-translational modifications: Autophosphorylated. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain. Database links: Entrez Gene: 10595 Human Entrez Gene: 2081 Human Entrez Gene: 78943 Mouse Entrez Gene: 498013 Rat Omim: 604033 Human SwissProt: O75460 Human SwissProt: Q76MJ5 Human SwissProt: Q9EQY0 Mouse SwissProt: Q9Z2E3 Mouse Unigene: 133982 Human Unigene: 592041 Human Unigene: 700027 Human Unigene: 20452 Mouse Unigene: 340943 Mouse Unigene: 226435 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
在酵母和哺乳动物细胞的内质直肠（ER）中未折叠蛋白的积累激活了未折叠蛋白反应（UPR）途径，并导致ER特异性基因转录参与蛋白折叠。UPR的激活需要ER跨膜激酶IRE1p（用于肌醇需要和ER到核的信号蛋白）。IRE1α和IRE1β是酵母IRE1p的两个哺乳动物同源物，这些相关蛋白定位于ER腔，并且包含跨越ER膜的短跨膜结构域和细胞浆Ser/Thr激酶结构域。IRE1的激活涉及蛋白质的细胞质部分的寡聚和反磷酸化，然后增强其固有的激酶活性，进而刺激UPR靶向基因的转录。针对应激反应，ER哺乳动物细胞的传感器激活IRE1α和IRE1β，然后导致JNK(Jun N-末端激酶)的磷酸化和细胞MAP激酶途径的激活。